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MicroStacker DETECTION SYSTEMS
MicroStacker™ Plus HRP-Polymer Detection Kit 
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MicroStacker™ Polymer Detection System is a biotin-free, polymeric horseradish peroxidase (HRP)- secondary antibody conjugate system for the detection of mouse and rabbit primary antibody on formalin-fixed, paraffin-embedded (FFPE) tissues in an immunohistochemistry (IHC) procedure. The innovative MicroStacker™ technology allows well-controlled layered stacking of antibodies and peroxidase enzymes on a micro-polymer scaffold. This technique results in a compact polymeric structure that easily penetrates to all cellular compartments. Plus, the system utilizes F(ab’) fragments of IgG secondary antibody instead of the whole IgG, which avoids the background caused by non-specific binding of whole IgG to endogenous Fc receptors. The degree of polymerization of enzymes and antibodies is upgraded with higher sensitivity. The optimal effect can be achieved simultaneously in the mouse/rabbit primary antibody. MicroStacker™ Plus HRP-polymer detection kit is suitable for various types of automatic immunohistochemistry stainer.
Data reviews: side-by-side comparison
Stainer Type：Bond Max
Staining Procedure：
Primary antibody incubation: RT, 30min
Linker: RT, 20min
Secondary antibody incubation: RT, 20min
DAB incubation: 5min
Detection polymers in comparison:
MicroStackerTM Plus
Bond Polymer Refine Detection
EnVision Flex+, Mouse Linker
Experimental Results:
Cervix (Ki67 mouse monoclonal antibody)
(MicroStackerTM Plus)
(EnVision Flex+)
(Bond Polymer Refine Detection)
|
Code |
Product Name |
Components |
Specification |
|
SD5600 |
MicroStackerTM Plus HRP-Polymer Detection Kit |
Peroxidase Blocking Reagents |
30ml*1 |
|
Linker, Rabbit-anti-Mouse |
30ml*1 |
||
|
Anti-Rabbit HRP-Polymer |
30ml*1 (300T) |
||
|
20*DAB Chromogen |
2.4ml*1 |
||
|
DAB Substrate Buffer |
30ml*2 |
||
|
Hematoxylin |
30ml*1 |
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