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MicroStacker DETECTION SYSTEMS
MicroStacker™ Flex HRP-Polymer Detection Kit (Mouse/Rabbit Linker) 
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Building 109, Headquarters Enterprise Base, No. 1 Cuizhu Street, High-tech Zone, Zhengzhou City, Henan Province, China
MicroStacker™ Polymer Detection System is a biotin-free, polymeric horseradish peroxidase (HRP)- secondary antibody conjugate system for the detection of mouse and rabbit primary antibody on formalin-fixed, paraffin-embedded (FFPE) tissues in an immunohistochemistry (IHC) procedure. The innovative MicroStacker™ technology allows well-controlled layered stacking of antibodies and peroxidase enzymes on a micro-polymer scaffold. This technique results in a compact polymeric structure that easily penetrates to all cellular compartments. Plus, the system utilizes F(ab’) fragments of IgG secondary antibody instead of the whole IgG, which avoids the background caused by non-specific binding of whole IgG to endogenous Fc receptors. According to your needs, the linker (mouse/rabbit) can be flexibly selected to further improve the staining effect. MicroStacker™ Flex HRP-polymer detection kit (mouse/rabbit linker) can be commonly used for detection of murine and rabbit antibodies. The Flex kit is suitable for various types of automatic immunohistochemistry stainer.
Data reviews: side-by-side comparison
Stainer Type：CNT330
Staining Procedure：
Primary antibody incubation: RT, 30min
Linker: RT, 20min
Secondary antibody incubation: RT, 20min
DAB incubation: 5min
Detection polymers in comparison:
MicroStackerTM Flex (Mouse Linker)
EnVision Flex+, Mouse Linker
Experimental Results:
Lymphoma (Ki67 mouse monoclonal antibody)
EnVision Flex+ 
MicroStackerTM FLex
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Product Name |
|
Format |
Components |
Specification |
|
MicroStackerTM Flex HRP-Polymer Detection Kit |
SD5100
|
V1
|
Peroxidase Blocking Reagents |
30ml*1 |
|
Linker, Rabbit-anti-Mouse |
30ml*1 |
|||
|
Anti-Rabbit HRP-Polymer |
30ml*1 (300T) |
|||
|
SD5300
|
V2
|
20*DAB Chromogen |
2.4ml*1 |
|
|
DAB Substrate Buffer |
30ml*2 |
|||
|
Hematoxylin |
30ml*1 |
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